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Part:BBa_M30333:Design

Designed by: Iny Jhun   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


Refactored M13 Between HpaI and BamHI Sites


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2177
    Illegal XbaI site found at 1260
    Illegal XbaI site found at 1587
    Illegal SpeI site found at 1601
    Illegal SpeI site found at 1725
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2177
    Illegal SpeI site found at 1601
    Illegal SpeI site found at 1725
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2177
    Illegal BamHI site found at 2898
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2177
    Illegal XbaI site found at 1260
    Illegal XbaI site found at 1587
    Illegal SpeI site found at 1601
    Illegal SpeI site found at 1725
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2177
    Illegal XbaI site found at 1260
    Illegal XbaI site found at 1587
    Illegal SpeI site found at 1601
    Illegal SpeI site found at 1725
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Overlaps between various genes were removed and enzymes were inserted between the separated genes, in order to allow for easier manipulation, such as deletion and insertion, of the genes.


Source

It comes from the M13K07 genome sequence.

References